在線咨詢:點擊這里給我發消息   點擊這里給我發消息
旺 旺:歡迎隨時給我旺旺留言
產品檢索
品名:
貨號:
品牌:
 
點擊排行
  您現在的位置:首頁 >> 特價優惠

Zeocin 特價促銷

發布人:浩然生物 瀏覽 33764次【字號 】 發布時間:2012年04月19日 打印本頁


品名產地貨號規格價格備注
Zeocin博萊霉素InvitrogenR250-011G31902500元優惠價,長年現貨
Zeocin博萊霉素InvitrogenR250-055G14410長年現貨
Zeocin博萊霉素InvitrogenR250-01125MG450長年現貨


    Zeocin可選擇Sh ble基因表達的細胞。與現在使用的其他動物細胞標記物無交叉抗性。因此這種抗生素可用來分離對其他篩選劑(如:慶大霉素,潮霉素)有抗性的克隆。Zeocin是一種屬于爭光霉素家族的糖蛋白抗生素,在體內能作用于大多數細菌(包括E。coli)、真菌(如:酵母菌)、植物細胞、動物細胞、細胞培養級! 
使用:
  ●液體Zeocin可用于細胞培養。
  ●用于篩選轉化株的Zeocin濃度根據pH值和鹽濃度改變。
  ●pH越高,鹽濃度越低Zeocin活性越大。
  ●普通大腸桿菌菌株篩選可在25µg/ml zeocin濃度的低鹽LB瓊脂培養基中進行,pH調至7.5。
  ●酵母篩選:取決于種屬和培養基,S。cerevisiae Sh ble轉化株在50µg/ml濃度的YPD培養基中篩選,pH7.0.304015.
    ●動物細胞篩選:根據不同細胞系從100到750µg/ml。
  ●植物細胞篩選:根據不同的細胞種類和培養基從50到250µg/ml! 
Invitrogen相關資料如下:
Cat. No. R250-01
Zeocin™ Selection Reagent
Description
Zeocin™ Reagent is a member of the bleomycin antibiotic family. Resistance is conferred by the Sh ble gene which encodes a 13,665 dalton protein. In cells expressing the protein, Zeocin™ is prevented from binding and cleaving the cellular DNA. Zeocin™ is effective on most aerobic cells and can be used for selection in mammalian and insect cell lines, yeast, and bacteria. The amount of Zeocin™ required for selection will vary between 50-2000 ug/ml (typically 300 ug/ml) depending on the cell type.
C55H83N19O21S2Cu
Formula Weight: 1137.41 g/mole
Zeocin™ is a trademark of CAYLA.
The ble resistance gene is the subject of one or more of U.S. Patent Nos. 5,021,344 and 5,118,620 and foreign equivalents owned by Cayla and licensed to Invitrogen. This product is sold for research purposes only. For commercial license information, please contact: Licensing Department, Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008.
General Specifications
Concentration:       100 mg⁄mL
Brand:       Zeocin
Form:       Liquid
Product Size:       1 g
Validated Application:       Eukaryotic Selection⁄Stable Cell Line Generation
Bacterial Selection
Contents & Storage
Zeocin™ is provided in solution at a concentration of 100 mg/ml. The 1 gram size is supplied in 8 vials. The 5 gram size is supplied in one vial. Store Zeocin™ at -20°C in the dark


有用 沒用    

上一個:GoldView I型核酸染色劑
下一個:GIBCO/Invitrogen 氨基酸
電話:021-54046790 傳真:021-54046791 郵箱:[email protected] [email protected] 地址:上海浩然生物技術有限公司 QQ:470003480
 Copyright © 2009上海浩然生物技術有限公司 All Rights Reserved. 網站備案:滬ICP備11023319號-1
福建31选7